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1.	Fibrinolytic Potency in Natto Food
2.	Natto: Anti-Oxidation
3.	Strong Fibrinolytic Activity of Nattokinase Lasting Longer Time Than An Ethical Drug
4.	Nattokinase Fibrinolytic Activity in Experimental Thrombus
5.	Lowering Blood Pressure and Cholesterol
6.	Anti-Bacterial Activity
7.	Thrombosis of the Central-Retina Vein
8.	Best Time to Eat Natto: Eat Natto at Supper and Continue to Eat It

1. Fibrinolytic Potency in Natto Food

	6 pcs of Natto (fermented soybeans) sold in a store were placed directly on a fibrin plate. 100g of Natto served in a pack for a meal has fibrinolytic activity equivalent to approx. 1600 IU/g of Urokinase.
	Comparative Test: (10 L each applied on a fibrin plate): N: Nattokinase extracted from 300g of natto P: Serum Plasmin standard 4.0 CU/mL U: Urokinase standard 100 IU/mL
	Nattokinase Heat-Resistance Test: Nattokinase extract was heat-treated at the temperature indicated (oC) for 10 minutes, and then applied on a fibrin plate. Each sample volume : 10 mL Incubation time: 18h at 37oC This test shows Nattokinase heat-resistance is up to 60oC.

2. Natto: Anti-Oxidation

Natto has stronger anti-oxidant effect than ordinary soybean. (Ezaki et al, 1992) Prof. Watanabe, U of Gifu revealed that anit-oxidant extracted from Natto cured stomach impairment caused by experimental stress in mice. This indicates that superoxide produced in peripheral blood vessels of stomach by stress was significantly suppressed.

3.

Strong Fibrinolytic Activity of Nattokinase Lasting Longer Time Than An Ethical Drug

	ELT(hr) Euglobulin Lysis Time	EFA(mm2) Euglobulin Fibrinolysis Time
Time after ingestion of Natto
0hr	31.56.2	0
2hr	16.48.6	8.45.1
4hr	16.76.6	15.23.1
8hr	19.312.0	5.84.1
12hr	27.410.3	1.95.2
24hr	31.598.9	0.80.6
Time after ingestion of boiled soybean
0hr	32.26.3	0
2hr	33.49.0	0
4hr	33.24.8	0
8hr	36.15.5	0
12hr	34.67.3	0
24hr	34.67.3	0.40.2

4. Nattokinase Fibrinolytic Activity in Experimental Thrombus

	Experimental thrombus in dog leg ( "circle" in the photo) was demonstrated.
4000IU of Nattokinase was orally administered.
	After 5 hours, lysis of the experimental thrombus was observed only with the dog administered.

5. Lowering Blood Pressure and Cholesterol

(1) Lowering Blood Pressure
	Volunteers who have high blood pressure were administered Nattokinase extracted from natto. Any medicine was administered in advance to the experiment. Arrows show the blood pressures after Nattokinase was administered. Both SBP (Systolic Blood Pressure) and DBP (Diastolic Blood Pressure) values (mmHg) were found in the normal blood pressure zone.
(2) Natto Lowers Cholesterol
Experiments in high-cholesterol-fed rabbits
	Cholesterol is a cause of high blood pressure. Natto is a high protein food like meat but contains no cholesterol. Furthermore natto contains lecithin that washes out cholesterol as well as linoleic acid that makes blood flow smoother. Anti-oxidant Vitamin E is also contained. It was proved that oxidation of fatty acid, especially oxidation of LDL- cholesterol was suppressed by natto. A person who cares about blood pressure is recommended to take 100g of natto 3~4 times a week in order to anticipate lowering effect in blood pressure.

6. Anti-Bacterial Activity

	Anti-bacterial components were extracted from natto sold in town. Standard agar-agar plate was left open for 5 minutes in air. Incubated for 2 days at 37oC. Left plate without natto extract shows colonies. Right plate shows no colony with natto extract.
l06pcs/ml of Bacillus subtilis of which concentration is found in a colon tract was added to 109 pcs/ml of O-157. On 4th day, O-157 disappeared.

7. Thrombosis of the Central-Retina Vein

	58 years old male, right eye Oral drug and hemostatic agent were administered while one pack of natto (100g) was served everynight. On 10th day bleeding from bottom eye was stopped. On 20th day, thrombosis was improved and vision was also recovered.
	Natto meal therapy was continued twice a week. After 2 months, thrombosis was cured completely. There was no side-effect reported. (Prof. Nishimura et al, University of Tottori, School of Medicine 1994)

8. Best Time to Eat Natto: Eat Natto at Supper and Continue to Eat It.

5 healthy volunteers ate 30 grams of natto on 1st day, and 30 grams on 2nd day, and 30 grams on 4th day. A peak of FDP was declining day after day. This indicates that thrombus in the blood vessels gradually has been dissolved. Reference: Sticky substance effect of Natto by Dr. H. Sumi, 1998 Furusato-Bunko Heart Publishing